DUET PhD in Biochemistry Question Paper with Answer Keys 2019-20 Download PDF

DUET PhD in Biochemistry Question Paper with Answer Keys 2019-20

DUET PhD in Biochemistry Question Paper 2019-20

Delhi University Entrance Test (DUET) 2019-20 PhD in Biochemistry Question Paper with answers DUET PhD in Biochemistry Question Paper 2019-20 with solution you can download it in FREE, if DUET Ph.D. in Biochemistry Question Paper 2019-20 in text or pdf for DUET PhD in Biochemistry Question Paper 2019-20 Answer Keys you can download DUET 2019-20 page also just Go to menu bar, Click on File->then Save.

DUET PhD in Biochemistry Question Paper with Answer Keys 2019-20

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EXAMPLE QUESTIONS

Q1. Transformation efficiency is defined as the number of colonies obtained upon transformation of one microgram of DNA. A student transformed 5 pg of DNA in DH5alpha cells and obtained 500 colonies. The transformation efficiency of DH5alpha cells is:

  • 1. 5 x 105
  • 2. 1 x 108
  • 3. 2.5 x 107
  • 4. 2.5 x 106

Q2. The half-life of 32P is 14.3 days. Approximately, how long would it take a solution containing 42,000 d.p.m to decay to 500 d.p.m?

  • 1. 2 months
  • 2. 60 days
  • 3. 100 days
  • 4. 90 days

Q3. If the egg white protein, ovalbumin, is denatured in a hard-boiled egg, then which of the following is least affected?

  • 1. The secondary structure of ovalbumin
  • 2. The primary structure of ovalbumin
  • 3. The quaternary structure of ovalbumin
  • 4. The tertiary structure of ovalbumin

Q4. Which of the following statements about heme structure is true?

  • 1. Heme contains a tetrapyrrole ring with four methyl, four vinyl, and four propionate side chains.
  • 2. The axial coordination positions of heme are occupied by tyrosine residues in myoglobin.
  • 3. The iron atom is coplanar with the tetrapyrrole ring in deoxyhemoglobin.
  • 4. The iron atom in heme may be present in ferrous or the ferric state.

Q5. A graduate student was trying to clone an insert digested with BssHII (G/CGCGC) and EcoRI enzyme (G/AATTC) in EcoRV (GAT/ATC) digested vector. Which of the following is the correct method for treatment of the insert to ensure its cloning into the vector?

  • 1. Treatment with T4 Polynucleotide kinase in presence of deoxynucleotides.
  • 2. Treatment with DpnI enzyme in the presence of deoxynucleotides.
  • 3. Treatment with T4 DNA polymerase in the presence of deoxynucleotides.
  • 4. Treatment with T4 DNA ligase in the presence of deoxynucleotides.

Q6. Length of a gene is 999 bases including the initiator and terminator codon. The number of amino acids in the protein encoded by this gene would be:

  • 1. 331
  • 2. 332
  • 3. 333
  • 4. 330

Q7. Glutathione does all of the following except

  • 1. Decrease the solubility of erythrocyte membrane
  • 2. Form conjugates with some drugs to increase water solubility
  • 3. Participate in the transport of amino acids across some cell membrane
  • 4. Scavenges peroxides

Q8. Many biosynthetic pathways are regulated by feedback inhibition of enzymes. In a particular type of feedback inhibition, two endproducts individually do not inhibit an enzyme upstream in the pathway at all, but when both are present they inhibit the same enzyme significantly. What type of feedback inhibition do these inhibitors represent?

  • 1. Additive inhibition
  • 2. Concerted inhibition
  • 3. Cumulative inhibition
  • 4. Synergistic inhibition

Q9. Griscelli syndrome is caused by mutation in :

  • 1. Rab27 protein involved in docking of transport vesicles
  • 2. Rab29 protein involved in formation of exocysts
  • 3. Sec12 protein involved in vesicle formation
  • 4. Atg 8 protein involved in autophagy

Q10. A mRNA coding for secretory protein, when translated using free ribosome under in vitro conditions, resulted in 40KDa protein. The same mRNA when translated using the rough ER resulted in a 36KDa protein. The difference in the molecular weight of the two polypeptides is due to the loss of a

  • 1. 4kDa peptide from the C-terminus
  • 2. 4kDa peptide from the N-terminus
  • 3. 2kDa protein from N-terminus and a 2kDa peptide from the C-terminus
  • 4. 1kDa peptide from N-terminus and a 3KDa peptide from the C-terminus

DUET PhD in Biochemistry Question Paper with Answer Keys 2019-20 Download PDF File:

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